31P NMR Studies of the Changes in Internal Inorganic Phosphate, ATP, Phosphocreatine and Phosphoethanolamine in Differentiated and Undifferentiated N1E-115 Neuroblastoma Cells Which Have Been Subjected to 80mM Extracellular KC1.

Neuroblastoma cells were seeded on Cytodex III microcarriers, put in the life-support system, which was put into a NMR 360 WB magnet and superfused with a growth medium supplemented with 10 mM creatine to insure that the cells were charged with phosphocreatine (PCr). KC1 was added to the recirculating superfusate of the life support system to a final concentration of 80mM.