A resonance Raman study of the temperature dependence of ligand photolysis and recombination in hemoglobins.
01 January 1984
Transient Raman spectroscopy has been used to study geminate recombination in photolyzed hemoglobins at cryogenic temperatures. The temperature dependence of the deoxy heme survival probability during a 10 nsec photolysis event is analyzed in terms of a distribution of energy barriers. Studies employing different ligands (CO, O2, NO), and a mutant hemoglobin (Hb-Zurich) demonstrates that the barriers controlling rebinding at early times are both ligand and protein specific.